diff --git a/src/main/java/htsjdk/samtools/SamInputResource.java b/src/main/java/htsjdk/samtools/SamInputResource.java
index d76694eeb..277c6e5fd 100644
--- a/src/main/java/htsjdk/samtools/SamInputResource.java
+++ b/src/main/java/htsjdk/samtools/SamInputResource.java
@@ -132,7 +132,13 @@ public SamInputResource index(final File file) {
 
     /** Updates the index to point at the provided resource, then returns itself. */
     public SamInputResource index(final Path path) {
-        this.index = new PathInputResource(path, Function.identity());
+        this.index = new PathInputResource(path);
+        return this;
+    }
+
+    /** Updates the index to point at the provided resource, with the provided wrapper, then returns itself. */
+    public SamInputResource index(final Path path, Function<SeekableByteChannel, SeekableByteChannel> wrapper) {
+        this.index = new PathInputResource(path, wrapper);
         return this;
     }
 
diff --git a/src/main/java/htsjdk/samtools/SamReaderFactory.java b/src/main/java/htsjdk/samtools/SamReaderFactory.java
index a5eef5038..8f203d5c0 100644
--- a/src/main/java/htsjdk/samtools/SamReaderFactory.java
+++ b/src/main/java/htsjdk/samtools/SamReaderFactory.java
@@ -81,10 +81,28 @@
 
     abstract public SamReader open(final File file);
 
+    /**
+     * Open the specified path (without using any wrappers).
+     *
+     * @param path the SAM or BAM file to open.
+     */
     public SamReader open(final Path path) {
-        final SamInputResource r = SamInputResource.of(path, getPathWrapper());
+        return open(path, null, null);
+    }
+
+    /**
+     * Open the specified path, using the specified wrappers for prefetching/caching.
+     *
+     * @param path the SAM or BAM file to open
+     * @param dataWrapper the wrapper for the data (or null for none)
+     * @param indexWrapper the wrapper for the index (or null for none)
+     */
+    public SamReader open(final Path path,
+            Function<SeekableByteChannel, SeekableByteChannel> dataWrapper,
+            Function<SeekableByteChannel, SeekableByteChannel> indexWrapper) {
+        final SamInputResource r = SamInputResource.of(path, dataWrapper);
         final Path indexMaybe = SamFiles.findIndex(path);
-        if (indexMaybe != null) r.index(indexMaybe);
+        if (indexMaybe != null) r.index(indexMaybe, indexWrapper);
         return open(r);
     }
 
@@ -106,25 +124,6 @@ public SamReader open(final Path path) {
     /** Sets a specific Option to a boolean value. * */
     abstract public SamReaderFactory setOption(final Option option, boolean value);
 
-    /** Sets a wrapper to modify the SeekableByteChannel from an opened Path, e.g. to add
-     * buffering or prefetching. This only works on Path inputs since we need a SeekableByteChannel.
-     *
-     * @param wrapper how to modify the SeekableByteChannel (Function.identity to unset)
-     * @return this
-     */
-    public SamReaderFactory setPathWrapper(Function<SeekableByteChannel, SeekableByteChannel> wrapper) {
-        this.pathWrapper = wrapper;
-        return this;
-    }
-
-    /** Gets the wrapper previously set via setPathWrapper.
-     *
-     * @return the wrapper.
-     */
-    public Function<SeekableByteChannel, SeekableByteChannel> getPathWrapper() {
-        return pathWrapper;
-    }
-
     /** Sets the specified reference sequence * */
     abstract public SamReaderFactory referenceSequence(File referenceSequence);
 
@@ -178,15 +177,10 @@ public static SamReaderFactory make() {
         private CRAMReferenceSource referenceSource;
 
         private SamReaderFactoryImpl(final EnumSet<Option> enabledOptions, final ValidationStringency validationStringency, final SAMRecordFactory samRecordFactory) {
-            this(enabledOptions, validationStringency, samRecordFactory, Function.identity());
-        }
-
-        private SamReaderFactoryImpl(final EnumSet<Option> enabledOptions, final ValidationStringency validationStringency, final SAMRecordFactory samRecordFactory, final Function<SeekableByteChannel, SeekableByteChannel> wrapper) {
             this.enabledOptions = EnumSet.copyOf(enabledOptions);
             this.samRecordFactory = samRecordFactory;
             this.validationStringency = validationStringency;
             this.customReaderFactory = CustomReaderFactory.getInstance();
-            setPathWrapper(wrapper);
         }
    
         @Override
@@ -417,7 +411,7 @@ private boolean isSra(final File sourceFile) {
         }
 
         public static SamReaderFactory copyOf(final SamReaderFactoryImpl target) {
-            return new SamReaderFactoryImpl(target.enabledOptions, target.validationStringency, target.samRecordFactory, target.getPathWrapper());
+            return new SamReaderFactoryImpl(target.enabledOptions, target.validationStringency, target.samRecordFactory);
         }
     }
 
diff --git a/src/test/java/htsjdk/samtools/PathInputResourceTest.java b/src/test/java/htsjdk/samtools/PathInputResourceTest.java
new file mode 100644
index 000000000..1bc2aa161
--- /dev/null
+++ b/src/test/java/htsjdk/samtools/PathInputResourceTest.java
@@ -0,0 +1,46 @@
+package htsjdk.samtools;
+
+import java.nio.channels.SeekableByteChannel;
+import java.nio.file.Path;
+import java.nio.file.Paths;
+import java.util.HashMap;
+import java.util.function.Function;
+import org.testng.Assert;
+import org.testng.annotations.Test;
+
+public class PathInputResourceTest {
+  final String localBam = "src/test/resources/htsjdk/samtools/BAMFileIndexTest/index_test.bam";
+
+  @Test
+  public void testWrappersAreAccessed() throws Exception {
+    Path path = Paths.get(localBam);
+    Path indexPath = Paths.get(localBam + ".bai");
+    HashMap<String, Boolean> fired = new HashMap<>();
+    Function<SeekableByteChannel, SeekableByteChannel> wrapData = (SeekableByteChannel in) -> {
+      fired.put("data", true);
+      return in;
+    };
+    Function<SeekableByteChannel, SeekableByteChannel> wrapIndex = (SeekableByteChannel in) -> {
+      fired.put("index", true);
+      return in;
+    };
+    SamInputResource in = SamInputResource.of(path, wrapData);
+    in.index(indexPath, wrapIndex);
+    InputResource indexResource = in.indexMaybe();
+    Assert.assertNotNull(indexResource);
+
+    Assert.assertFalse(fired.containsKey("data"));
+    Assert.assertFalse(fired.containsKey("index"));
+
+    indexResource.asUnbufferedSeekableStream();
+
+    Assert.assertFalse(fired.containsKey("data"));
+    Assert.assertTrue(fired.containsKey("index"));
+
+    in.data().asUnbufferedSeekableStream();
+
+    Assert.assertTrue(fired.containsKey("data"));
+    Assert.assertTrue(fired.containsKey("index"));
+  }
+
+}
diff --git a/src/test/java/htsjdk/samtools/SamReaderFactoryTest.java b/src/test/java/htsjdk/samtools/SamReaderFactoryTest.java
index 4c045d9d8..1d5163346 100644
--- a/src/test/java/htsjdk/samtools/SamReaderFactoryTest.java
+++ b/src/test/java/htsjdk/samtools/SamReaderFactoryTest.java
@@ -6,6 +6,7 @@
 import htsjdk.samtools.seekablestream.SeekableHTTPStream;
 import htsjdk.samtools.seekablestream.SeekableStreamFactory;
 import htsjdk.samtools.util.*;
+import java.net.URI;
 import java.nio.ByteBuffer;
 import java.nio.channels.SeekableByteChannel;
 import java.nio.file.Paths;
@@ -92,8 +93,7 @@ public void testWrap() throws IOException {
         final SamReader wrappedReader =
             SamReaderFactory
                 .makeDefault()
-                .setPathWrapper(SamReaderFactoryTest::addHeader)
-                .open(input);
+                .open(input, SamReaderFactoryTest::addHeader, null);
         int records = countRecords(wrappedReader);
         Assert.assertEquals(10, records);
     }
@@ -289,7 +289,6 @@ public void openPath() throws IOException {
         }
     }
 
-
     final Set<List<SAMRecord>> observedRecordOrdering1 = new HashSet<List<SAMRecord>>();
     final Set<List<SAMRecord>> observedRecordOrdering3 = new HashSet<List<SAMRecord>>();
     final Set<List<SAMRecord>> observedRecordOrdering20 = new HashSet<List<SAMRecord>>();
diff --git a/src/test/java/htsjdk/samtools/SamReaderTest.java b/src/test/java/htsjdk/samtools/SamReaderTest.java
index 093dffbeb..02364f318 100644
--- a/src/test/java/htsjdk/samtools/SamReaderTest.java
+++ b/src/test/java/htsjdk/samtools/SamReaderTest.java
@@ -23,7 +23,6 @@
  */
 package htsjdk.samtools;
 
-import htsjdk.samtools.cram.CRAMException;
 import htsjdk.samtools.util.CloseableIterator;
 import htsjdk.samtools.util.CloserUtil;
 import org.testng.Assert;